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- W2356868082 abstract "Objective To clone and express the chitinase gene from Bombyx mori Linaeus in prokaryotic system. Methods The RT-PCR using gene specific primers was applied to clone the full-length gene of chitinase gene from Bombyx mori Linaeus and then the sequences were analyzed. The ORF of chitinase gene of Bombyx mori Linaeus was subcloned into the expression vector pET-28a. Results Chitinase gene from Bombyx mori Linaeus was cloned and constructed to prokaryotic expression vector. The cloned cDNA ORF sequence contained 1 668 bp and encoded 555 amino acids. Sequence analysis showed that the estimated molecular mass is 61.5 kD,pI 5.78. The cloned sequence is 99% identical with chitinase gene from Bombyx mori Linaeus. Conclusion Chitinase gene from Bombyx mori Linaeus was cloned and constructed to the prokaryotic expression vector successfully. It establishes the foundation for the recombinant allergen chitinase expression and the activity studies." @default.
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- W2356868082 date "2010-01-01" @default.
- W2356868082 modified "2023-09-24" @default.
- W2356868082 title "Cloning and Prokaryotic Expression of Chitinase Gene from Bombyx mori Linaeus" @default.
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