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- W2356973000 abstract "To establish a rapid and effective quarantine method of the Kio Hepesvirus disease,a set of primers and TaqMan probe for Fluorescent Quantitation PCR were designed to detect the conservative sequence of Kio Hepesvirus Sph genes.The cell cultures were detected by using the established quantitative PCR assay,and the results were compared with those of the routine PCR.The quantitative PCR sensitivity was higher than that of the routine PCR.The quantity of KHV DNA was 1.6×102 copies/μL.A reliable diagnostic result can be obtained just within 4 h.The assay proved to be a rapid,sensitive,specific and repetitive method for rapid detection of Kio Hepesvirus from fish in quarantine." @default.
- W2356973000 created "2016-06-24" @default.
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- W2356973000 date "2012-01-01" @default.
- W2356973000 modified "2023-09-25" @default.
- W2356973000 title "Establishment and Application of the TaqMan Real-Time PCR Detection Assay for Koi Herpesvirus" @default.
- W2356973000 hasPublicationYear "2012" @default.
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