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- W2356990834 abstract "The fluorescence quenching method was applied to study the combination effects of sulfonamides(SAs), trimethoprim(TMP) and fish serum albumin(FSA). Meanwhile, the influence of the protein on extraction of SAs and TMP was investigated in detail by HPLC. The results showed that SAs and TMP strongly bound with FSA. In 80%(V/V) tetrahydrofuran aqueous solution, a slow but full protein denaturation took place, which would cause the unfolding of protein and release of drug residues. When K2HPO4 was added to the extractant of tetrahydrofuran solution,the aqueous two phase system(ATPS) was formed. The liposoluble drug residues were extracted to the upper phase of ATPS with high extraction efficiencies. Meanwhile, the fat-soluble impurities are extracted less in 80%(V/V) tetrahydrofuran aqueous solution than those in hydrophobic solvents as liquid-liquid extraction procedure. Therefore the purification steps were greatly simplified. The proposed approach was satisfactorily applied to the quick determination of SAs and TMP residues in fish by HPLC. Overall recoveries were 80.9%-95.1% with RSD values of 1.9%-5.9%, and the detection limits were in the range of 6.3-23.7 ng/g." @default.
- W2356990834 created "2016-06-24" @default.
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- W2356990834 date "2013-01-01" @default.
- W2356990834 modified "2023-09-24" @default.
- W2356990834 title "Optimization of pre-treatment methods of sulfonamides residues in fish by HPLC" @default.
- W2356990834 hasPublicationYear "2013" @default.
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