Matches in SemOpenAlex for { <https://semopenalex.org/work/W2357001490> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2357001490 abstract "The genomic DNA of Pseudorabies virus(PRV) Ea strain was extracted,purified and digested with BamHⅠ.The 4 8 kb fragments were recovered and cloned into the vector pUC18,resulting in lots of recombinant plasmids consisted of different 4 8 kb BamHⅠ fragments.A recombinant plasmid pUCIE contained the complete IE180 gene was selected by restriction analysis,sequence determination and comparison with the IE180 gene of other PRV strain.Then,a 1 8 kb fragment encoding the N terminus of IE180 was subcloned into downstream of the T7 promoter of the expression vector,pET 28a,to yield the recombinant expression plasmid pET1 8.After transformed into E.coli BL21(DE3),fusion protein was expressed under the induction with IPTG.The results of SDS PAGE analysis showed that the expressed protein was 62 000 in size and formed inclusion body.The specificities of the fusion protein was also confirmed to react with the antiserum against 6×His by Western immunoblotting assay.The expression products would be useful to further study the structure and function of the IE180 of PRV." @default.
- W2357001490 created "2016-06-24" @default.
- W2357001490 creator A5007190828 @default.
- W2357001490 date "2003-01-01" @default.
- W2357001490 modified "2023-09-27" @default.
- W2357001490 title "Cloning of the Immediate-early Gene IE180 of Pseudorabies Virus Ea Strain and Its Expression in E.coli" @default.
- W2357001490 hasPublicationYear "2003" @default.
- W2357001490 type Work @default.
- W2357001490 sameAs 2357001490 @default.
- W2357001490 citedByCount "1" @default.
- W2357001490 crossrefType "journal-article" @default.
- W2357001490 hasAuthorship W2357001490A5007190828 @default.
- W2357001490 hasConcept C104317684 @default.
- W2357001490 hasConcept C107618985 @default.
- W2357001490 hasConcept C121050878 @default.
- W2357001490 hasConcept C123894998 @default.
- W2357001490 hasConcept C128319531 @default.
- W2357001490 hasConcept C150194340 @default.
- W2357001490 hasConcept C153911025 @default.
- W2357001490 hasConcept C159047783 @default.
- W2357001490 hasConcept C159654299 @default.
- W2357001490 hasConcept C170344550 @default.
- W2357001490 hasConcept C19924922 @default.
- W2357001490 hasConcept C199360897 @default.
- W2357001490 hasConcept C22744801 @default.
- W2357001490 hasConcept C2522874641 @default.
- W2357001490 hasConcept C2781092067 @default.
- W2357001490 hasConcept C33161422 @default.
- W2357001490 hasConcept C40767141 @default.
- W2357001490 hasConcept C41008148 @default.
- W2357001490 hasConcept C54355233 @default.
- W2357001490 hasConcept C86803240 @default.
- W2357001490 hasConcept C91523082 @default.
- W2357001490 hasConceptScore W2357001490C104317684 @default.
- W2357001490 hasConceptScore W2357001490C107618985 @default.
- W2357001490 hasConceptScore W2357001490C121050878 @default.
- W2357001490 hasConceptScore W2357001490C123894998 @default.
- W2357001490 hasConceptScore W2357001490C128319531 @default.
- W2357001490 hasConceptScore W2357001490C150194340 @default.
- W2357001490 hasConceptScore W2357001490C153911025 @default.
- W2357001490 hasConceptScore W2357001490C159047783 @default.
- W2357001490 hasConceptScore W2357001490C159654299 @default.
- W2357001490 hasConceptScore W2357001490C170344550 @default.
- W2357001490 hasConceptScore W2357001490C19924922 @default.
- W2357001490 hasConceptScore W2357001490C199360897 @default.
- W2357001490 hasConceptScore W2357001490C22744801 @default.
- W2357001490 hasConceptScore W2357001490C2522874641 @default.
- W2357001490 hasConceptScore W2357001490C2781092067 @default.
- W2357001490 hasConceptScore W2357001490C33161422 @default.
- W2357001490 hasConceptScore W2357001490C40767141 @default.
- W2357001490 hasConceptScore W2357001490C41008148 @default.
- W2357001490 hasConceptScore W2357001490C54355233 @default.
- W2357001490 hasConceptScore W2357001490C86803240 @default.
- W2357001490 hasConceptScore W2357001490C91523082 @default.
- W2357001490 hasLocation W23570014901 @default.
- W2357001490 hasOpenAccess W2357001490 @default.
- W2357001490 hasPrimaryLocation W23570014901 @default.
- W2357001490 hasRelatedWork W1986628811 @default.
- W2357001490 hasRelatedWork W2114833873 @default.
- W2357001490 hasRelatedWork W2348876289 @default.
- W2357001490 hasRelatedWork W2349741016 @default.
- W2357001490 hasRelatedWork W2352490840 @default.
- W2357001490 hasRelatedWork W2354932289 @default.
- W2357001490 hasRelatedWork W2356656275 @default.
- W2357001490 hasRelatedWork W2362415804 @default.
- W2357001490 hasRelatedWork W2364445136 @default.
- W2357001490 hasRelatedWork W2367970853 @default.
- W2357001490 hasRelatedWork W2368904088 @default.
- W2357001490 hasRelatedWork W2371406579 @default.
- W2357001490 hasRelatedWork W2372005558 @default.
- W2357001490 hasRelatedWork W2376049804 @default.
- W2357001490 hasRelatedWork W2376821269 @default.
- W2357001490 hasRelatedWork W2381069110 @default.
- W2357001490 hasRelatedWork W2381910036 @default.
- W2357001490 hasRelatedWork W2383047217 @default.
- W2357001490 hasRelatedWork W2389244756 @default.
- W2357001490 hasRelatedWork W2908621633 @default.
- W2357001490 isParatext "false" @default.
- W2357001490 isRetracted "false" @default.
- W2357001490 magId "2357001490" @default.
- W2357001490 workType "article" @default.