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- W2357004607 abstract "The total RNA was extracted from normal sika deer antler hypertrophic chondrocyte and used as a template for RT-PCR.A 2 038 bp fragment containing the whole length gene coding region of protein colX was obtained and cloned into pMD18-T vector.The recombinant which had analysied by restriction endonucleases and PCR was sequenced to confirm its identity.The right recombinant was ligated with vector pET-28a(+) and transformed into E.coli BL21(DE3).After being induced by IPTG,SDS-PAGE and Western-blot analysis confirmed that the transformed E.coli BL21 could express colX proteins with the molecular weight of 65 000.The results showed that the sika deer colX protein was obtained successfully." @default.
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- W2357004607 date "2008-01-01" @default.
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- W2357004607 title "Cloning and prokaryotic expression of sika deer colX gene" @default.
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