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- W2357014794 abstract "Objective To study the differentiation of neural stem cells(NSCs) and their electrophysiological properties after neural differentiation treated with olfactory ensheathing cells(OECs).Methods NSCs cultured in DMEM/F12 serum-free medium were induced to differentiate to neurons by adding OEC-conditioned medium.Nestin was used to identify NSCs and neurofilament 200(NF200) was used to identify neurons.The patch clamp was used to detect the electrophysiological properties of differentiated neurons.ResultsOECs could promote the differentiation of NSCs into neurons.Some neurons displayed fast etrodotoxin(TTX)-sensitive Na+ current,as well as slow tetraethylammonium chloride(TEA)-sensitive delayed-rectifier K+ current.Conclusion OECs can promote the differentiation of NSCs.The NSCs-differentiated neurons have active electrophysiological properties,which are potential to replace the apoptotic or necrotic neurons." @default.
- W2357014794 created "2016-06-24" @default.
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- W2357014794 date "2010-01-01" @default.
- W2357014794 modified "2023-09-25" @default.
- W2357014794 title "Differentiation of neural stem cells and their electrophysiological properties after neural differentiation treated with olfactory ensheathing cells" @default.
- W2357014794 hasPublicationYear "2010" @default.
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