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- W2357021189 abstract "Objective: To extract,separate and identify anti-A549 cell constituents from thermally processed Gynostemma pentaphyllum leaves.Methods: Gynostemma pentaphyllum leaves were processed at 125 ℃ and 0.24 MPa for 3 h and subjected to heat reflux extraction with 80% ethanol for 3 h.Anti-A549 cell constituents in the extract were separated using HP-20 macroporous adsorption resin and a reversed-phase column by means of bioassay-guided separation and identified by1H-NMR,13C-NMR and ESI-MS.Results: Two dammarane-type saponins were obtained and identified as gypenoside L and gypenoside LI,respectively.Both gypenosides had a concentration-dependent inhibitory effect on A549 cells with IC50 values of(48.43 ± 1.33) μg/mL and(34.35 ± 0.88) μg/mL,respectively.Conclusion: Thermally processed Gynostemma pentaphyllum leaves have a notable inhibitory effect on A549 cells,which is associated with the presence of gypenoside L and gypenoside LI." @default.
- W2357021189 created "2016-06-24" @default.
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- W2357021189 date "2012-01-01" @default.
- W2357021189 modified "2023-09-22" @default.
- W2357021189 title "Purification, Identification and Anti-A549 Cell Activity of Gypenosides from Thermally Processed Gynostemma pentaphyllum Leaves" @default.
- W2357021189 hasPublicationYear "2012" @default.
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