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- W2357089519 abstract "Objective To investigate the biological character changes of keratinocyte proliferated in vitro and to investigate the relationship between growth arrest and keratin19 mRNA expression and clonal forming efficiency. Methods The human foreskin was obtained from 20 patients, and the keratinocytes were isolated by 2.4 U/mL dis-pase and 0.05% tripsin digestion, and then cultured in vitro. Growth curve was made from data of cell counting. Cell phenotype was observed during cell culture under light microscope. Meanwhile colonal analysis was also performed. The mRNA expression of keratin 19 and involucrin was detected by RT-PCR. Results The children foreskin keratinocyte could be expanded for 700 ±37 times. RT-PCR results showed that K19 mRNA expressed at passages 0,2,4,5 vanished at passage6 ; involucrin expressed at all passages. Clonal forming efficiency is (45±4) % , (37 ±4)%,(28±3)%,(9±2)% at passage 0,2,4,5 respectively and vanished at passage6. Conclusion During expansion of keratinocytes, the ability of proliferation reduces. Growth arrest may be relative to decreased clony forming efficiency and K19 mRNA expression." @default.
- W2357089519 created "2016-06-24" @default.
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- W2357089519 date "2004-01-01" @default.
- W2357089519 modified "2023-09-24" @default.
- W2357089519 title "Proliferation and biological character changes of human keratinocytes cultured in vitro" @default.
- W2357089519 hasPublicationYear "2004" @default.
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