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- W2357100965 abstract "Aim: To amplificate,clone and sequence the thymidine kinase (TK) gene of herpes simplex virus 1(HSV I). Methods:The BHK21 cells were infected by HSV I 17 syn + strain. HSV I genomic DNA was purified from the BHK 21 cells suspension and used as template to run PCR for TK gene amplification. The amplificated products were cloned into pUC 18 vector and sequenced. Results: Coding region of HSV 1 TK gene consisted of 1128 bp except stop code, it encoded 376 amino acids. Among the 1128 bp, there were 12 Met and 4 Cys. The 249 and 250 amino acid residues of TK were Leu and Gln respectively. Their corresponding codes were CTG and GAG which formed one Pst1 site. The 313 and 314 amino acid residues were Asp and Val respectively. Their corresponding codes were GAC and GTC which formed another PstI site. Conclusion:The coding sequence amplificated in this study is the entire sequence of coding region of HSV 1 TK." @default.
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- W2357100965 date "1998-01-01" @default.
- W2357100965 modified "2023-09-24" @default.
- W2357100965 title "Amplification,cloning and sequencing of the thymidine kinase gene of herpes simplex virus I" @default.
- W2357100965 hasPublicationYear "1998" @default.
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