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- W2357104921 abstract "We established a rapid detection method of Haemophilus spp.in tree shrews,and provided a reference for tree shrews microbiological quality control.According to the outer membrane protein(OMP)multiple alignments of 6different Haemophilus spp.published in NCBI,a few pairs of degenerate primers were designed by CODEHOP designer online.Then,the specificity and sensitivity of designed CODEHOPs were evaluated by 4standard Haemophilus strains and 24 other genus strains.And Haemophilus spp.in the tree shrews was detected by established CODEHOP PCR method.Results showed that degenerate primers HF-1/HR-8and HF-3/HR3 could produce target fragments of 506-535 bp and 344-390 bp by amplifying H.paragallinarum(ATCC29545),H.haemolyticus(ATCC33390),H.influenzae(ATCC33391),H.parainfluenzae(ATCC33392),respectively.Amplified nucleotides were sequenced and Blast in NCBI,which proved that results were accurate.It can effectively distinguish Haemophilus spp.and other genus together with two pairs of primers.The lowest detection limit of this method was 2.1pg/uL.The 60 respiratory specimens of wild tree shrews were tested for Haemophilus spp.that positive rate was 20/60.In conclusion,the CODEHOP PCR performs well for detection Haemophilus spp.in animal clinical specimens.It is easy to operate,with good specificity and sensitivity,and has practical application value." @default.
- W2357104921 created "2016-06-24" @default.
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- W2357104921 date "2015-01-01" @default.
- W2357104921 modified "2023-09-27" @default.
- W2357104921 title "Establishment and application of CODEHOP PCR assay for detection of Haemophilus spp.in tree shrews" @default.
- W2357104921 hasPublicationYear "2015" @default.
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