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- W2357129153 abstract "PurposeIn an attempt to make a comparison between the collagens from E.coli expression and bovine tendon in terms of amino acid composition,secondary molecular conformation,and thermal properties.MethodsThe bovine tendon collagen type Ⅰ(BTCg) was isolated and purified by means of limited pepsin digestion and salt precipitation.Amino acid contents of both the recombinant human-like collagen(RHCg) and the BTCg were assayed by an amino acid analyzer.Molecular secondary structures of the RHCg and the BTCg in solution were examined using a circular dichroism(CD) before and after denaturation.Thermal behaviors of both were examined using a differential scanning calorimetry(DSC),a modulated DSC as well as a thermogravimetry analysis(TGA).ResultsThe RHCg had a significant different amino acid composition in comparison with that of the BTCg.Especially,for example,the former contained no hydroxyproline,a low percentage of imino acid(the RHCg,17.83% residue,the BTCg,22.03% residue),lysine(the RHCg,0.90% residue,the BTCg,2.37% residue),arginine(the RHCg,1.01% residue,the BTCg,5.33% residue),and glutamic acid(the RHCg,8.13% residue,the BTCg,12.91% residue).Molecular secondary structures of the RHCg and the BTCg both posed poly-L-proline conformation(P-Ⅱ) in solution at 20 ℃.The RHCg showed a different thermal behavior between 140-280 ℃ compared with that of the BTCg.ConclusionAlthough the RHCg and the BTCg were both in P-Ⅱ conformation in solution before denaturation,they had different amino acid compositions,and showed significant different thermal properties.Accordingly,thorough understanding the characteristic of collagens from different sources is of importance for rational engineering and application of collagen-based devices." @default.
- W2357129153 created "2016-06-24" @default.
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- W2357129153 date "2006-01-01" @default.
- W2357129153 modified "2023-10-05" @default.
- W2357129153 title "A comparison between collagens from E.coli expression and bovine tendon" @default.
- W2357129153 hasPublicationYear "2006" @default.
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