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- W2357147199 abstract "The nematode resistant gene N is a single dominant gene in pepper cultivar Carolina Wonder(Capsicum annuum L.).To investigate the early up-regulated expression genes profile in cultivar Carolina Wonder induced by root-knot nematode(Meloidogyne incognita,RKN),a forward subtracted cDNA library was constructed using suppression subtractive hybridization(SSH).The cDNA of pepper seedling root tips inoculated with J2 RKN for 12,24 and 36 h were used as tester and that from untreated root tips as driver.Totally 237 RKN-induced SSH cDNA fragments were selected with DIG Nonradioactive Nucleic Acid Labeling and Detection System.Based on the sequencing and BLAST analyzing of 148 fragments,68 up-regulated expression EST fragments were identified,including the genes encode nucleotide-binding site/leucine-rich repeat(NBS-LRR) disease resistant proteins and LRR resistance protein-like;some defense proteins,such as Germin-like protein,HSR203J protein-like,Nectarin,and Snakin-2;some biostress resistance related transcription factor like WRKY and ERFBP;some signal proteins,such as GRP-like protein and 14-3-3 family protein.The Gene Ontology analysis revealed that the up-regulated ESTs involved in the recognition to nematode,signal transduction,protection mechanism,HR and SAR.In addition,some ESTs of unknown function may be novel genes." @default.
- W2357147199 created "2016-06-24" @default.
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- W2357147199 date "2007-01-01" @default.
- W2357147199 modified "2023-09-25" @default.
- W2357147199 title "Analysis of Early Expression Genes Resistance to Root Knot Nematode in N Gene Pepper by SSH" @default.
- W2357147199 hasPublicationYear "2007" @default.
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