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- W2357153485 abstract "Objective To determine whether direct intra-myocardial injection of naked DNA en- coding for vascular endothelial growth factor could enhance collateral circulation in a rabbit model of acute myocardial ischemin.Methods A rabbit model of acute myocardial ischemia was made by ligation of left main coronary artery.The pcDNA3/hVEGD_(165)and pcDNA3 were injected into the border zone of the is- chemic myocardium.Two and 4-weeks later the left ventricular myocardium was taken for analysis of his- tologic studies,RT-PCR and protein concentration.Results RT-PCR and protein concentration analysis revealed that the expression of hVEGD_(165)mRNA and VEGF protein in VEGF gene group was higher then that in the control group on the 4th week.HE and VEGF immunostaining confirmed that the densi- ties of vessels in ischemic areas were obviously increased in experimental group as compared with,that in control group.Conclusion VEGF gene enhances the blood vessel counts and accelerates the development of collateral circulation in ischemic myocardium." @default.
- W2357153485 created "2016-06-24" @default.
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- W2357153485 date "2006-01-01" @default.
- W2357153485 modified "2023-09-26" @default.
- W2357153485 title "Directed gene transfection with vascular endothelial growth factor enhances collateral circulation in ischemic myocardium" @default.
- W2357153485 hasPublicationYear "2006" @default.
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