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- W2357188844 abstract "Several inoculation methods were compared to test their effect of pathogenicity to Stylosanthes guianensias. In all 1 230 mutants, 23 had decreased weak pathogenicity, 5 even completely losted its ability to infect S. guianensias. TAIL-PCR was used to identify the T-DNA integration site in the mutant that was coded t-2430 and the sequence of 476 bp was gotten. The flanking sequence of T-DNA was compared with Colletotrichum genome by blast and 401nt of sequence was found in Contig464 of Colletotrichum genome. By predicting the function of flanking sequence, we found T-DNA insertion in the promoter region of putative gene, which had 79% homology to Magnaporthe oryzae aspartate aminotransferase gene(XP_003719674.1)." @default.
- W2357188844 created "2016-06-24" @default.
- W2357188844 creator A5058777884 @default.
- W2357188844 date "2014-01-01" @default.
- W2357188844 modified "2023-09-26" @default.
- W2357188844 title "Screening of pathogenicity defect mutant in Colletotrichum gloeosporioides of Stylosanthes guianensias and molecular cloning of T-DNA integration flanking sequence" @default.
- W2357188844 hasPublicationYear "2014" @default.
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