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- W2357202293 abstract "Objective:To establish a HPLC assay for human plasma vancomycin. Methods:The plasma vancomycin extracted by the sedimentation with 10% ZnSO_4 was assayed through thermo-C_(18) column (250mm×4.6mm,5μm) and a mixture of methanol and KH_2PO_4 buffer solution (20:80) (pH 3.2). Other HPLC conditions were optimized below: the elution rate of 1.0mL·min~(-1), the sample loading volume of 10μL,the column temperature at 25℃ and the detection at 230nm. Results:The detection limit for vancomycin was 80 ng·mL~(-1). The calibrated linear plot of vancomycin was within 5.0～80.00mg·L~(-1)(r=0.9947,n=5) with the relevant recovery within 98.67%～100.40% and within-day and among-days RSD within 1.6%～3.4%. Conclusion: The sensitive and reproducible HPLC conditions may be used in blood concentration monitoring and pharmacokinetics study for vancomycin." @default.
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- W2357202293 date "2005-01-01" @default.
- W2357202293 modified "2023-09-23" @default.
- W2357202293 title "Determination of plasma vancomycin by HPLC" @default.
- W2357202293 hasPublicationYear "2005" @default.
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