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- W2357202295 abstract "Carbofuran degrading mutant CDS-M1 was obtained by mutating Sphingomonas agrestis CDS-1 with transposon Tn5 carried on the suicide plasmid pSC123.Genomic DNA library was constructed in E.coli DH5α using pMD18-T as vector.By using transposon rescue,a 4 551 bp DNA sequence of S.agrestis CDS-1 flanking the Tn5 insertion site was obtained.A pair of PCR primers were synthesized according to the sequence adjacent to transposon.With these primers,a same size PCR product was amplified from the genomic DNA of CDS-1.The PCR product was ligated into broad host vector pPZP201 to construct recombined plasmid CDZ1.CDZ1 was then transferred into mutant CDS-M1 by triparental mating.The result showed that the transformant with CDZ1 had regained carbofuran degrading ability.The study indicated that the 4 551 bp DNA sequence was a gene fragment relative to carbofuran hydrolyzing.Fig 7,Tab 1,Ref 14" @default.
- W2357202295 created "2016-06-24" @default.
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- W2357202295 date "2009-01-01" @default.
- W2357202295 modified "2023-09-25" @default.
- W2357202295 title "Cloning of a Gene Related to Carbofuran Hydrolyzing from Sphingomonas agrestis CDS-1 by Transposon Rescue" @default.
- W2357202295 hasPublicationYear "2009" @default.
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