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- W2357206994 abstract "Objective To construct siRNA recombinant expression vector targeting HIF-1α gene.Methods Genome sequences of HIF-1α gene were retrieved from gene bank,and four pairs of oligonucleotides were synthesized and inserted into plasmid pGensil-1.Then,DH5α strains were transformed,and plasma was extracted and identified by restriction endonuclease and sequence analysis.Results It was confirmed by sequence analysis that siRNA recombinant expression vector targeting HIF-1α gene coincided completely with the designs.Conclusions The siRNA recombinant expression vector targeting HIF-1α gene has been constructed successfully and lays a foundation for furher study on the biological behavior of tumors." @default.
- W2357206994 created "2016-06-24" @default.
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- W2357206994 date "2010-01-01" @default.
- W2357206994 modified "2023-09-22" @default.
- W2357206994 title "Construction and identificication of siRNA recombinant expression vector targeting HIF-1α gene" @default.
- W2357206994 hasPublicationYear "2010" @default.
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