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- W2357238840 abstract "Objective To investigate a new method for T/B cell simultaneous depletion from mobilized peripheral blood stem cell graft in systemic lupus erthymatosus. Methods In this study, we deplete T/B cell using anti-CD3 and anti-CD19 antibodies conjugated to magnetic microbeads by the CliniMACS device. Flow cytometry were performed in order to determine the depletion efficiency of T-cell and B-cells. Colony assays were also used to evaluate the function of the stem cells after depletion. Results The CliniMACS system can be used to efficiently deplete T and B cells from PBSCs simultaneously, without adverse effect on biological function of hematopoietic stem cells according to colony assays. Conclusion Our data suggest that the CliniMACS system is a quite effective method to deplete T and B cells from PBSCs simultaneously, and play a significant role in decreasing the risk of recurrence and EBV-associated lymphoproliferative disease." @default.
- W2357238840 created "2016-06-24" @default.
- W2357238840 creator A5006226566 @default.
- W2357238840 date "2013-01-01" @default.
- W2357238840 modified "2023-09-27" @default.
- W2357238840 title "Efficient of T/B Cell Depletion from Mobilized Peripheral Blood Stem Cell Graft in Systemic Lupus Erthymatosus" @default.
- W2357238840 hasPublicationYear "2013" @default.
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