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- W2357245042 abstract "The capsid p27 gag gene of SIVmac239 was cloned into the pET32a (+) and expressed in E.coli BL21. The fusion protein was purified by Ni2+ affinity chromatography and used as antigen for monolconal antibody development. Five hybridoma cell lines that secreted monoclonal antibody (mAbs) against p27 were established and were designated as 1A5, 1C7, 3C2, 2D12 and 3D12. All these five mAbs were IgG1 subtype and consisted of λ light chain. The titers of mAbs in ascites were 1∶12 800, 1∶25 600, 1∶819 200, 1∶51 200 and 1∶409 600, respectively. Furthermore, all these five mAbs reacted specifically with the capsid p27 protein of SHIV89.6P in Western blot and IFA." @default.
- W2357245042 created "2016-06-24" @default.
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- W2357245042 date "2008-01-01" @default.
- W2357245042 modified "2023-09-23" @default.
- W2357245042 title "Development of monoclonal antibodies against the capsid p27 protein of SIV_(mac239)" @default.
- W2357245042 hasPublicationYear "2008" @default.
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