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- W2357245086 abstract "In this study,11 species of lactic acid bacteria were isolated from milk samples,which were collected from Inner Mongolia and Qinghai.The general investigation,elimination and stability of the endogenous plasmid DNA of these bacteria were carried out by nucleic acid electrophoresis,medicine sensitive test and SDS-variable temperature elimination.The results showed that all of the 11 species had the suspicious bands of plasmid DNA in different sizes.And these bands could be divided into two types,the one was larger than 23 kb and the other was smaller than 23 kb.3 species were fallen into disuse because they could not grow after their plasmid DNA was eliminated by SDS-variable temperature elimination.In the residual 8 species of lactic acids bacteria,the smaller bands were completely eliminated by the method of SDS-variable temperature elimination and a few of the larger bands could be eliminated too.The above results corresponded with the medicine sensitive subsidiary test.The 8 species of lactic acid bacteria that had been eliminated the plasmid DNA presented the good hereditary stability after being cultured 20 generations.This study has laid the foundation for the screening and establishing new genetic engineering host." @default.
- W2357245086 created "2016-06-24" @default.
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- W2357245086 date "2008-01-01" @default.
- W2357245086 modified "2023-09-25" @default.
- W2357245086 title "General investigation and elimination of the endogenous plasmid DNA of edible lactic acid bacteria and their stability test" @default.
- W2357245086 hasPublicationYear "2008" @default.
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