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- W2357282652 abstract "AIM:To study the role of extracellular signal regulated kinase(ERK) pathway in cocaine-and amphetamine-regulated transcript(CART) induced brain-derived neurotrophic factor(BDNF) synthesis in primary cultured hippocampal neurons.METHODS:The primary cultures of hippocampal neurons were established from embryonic rats(day 18 of gestation).The neurons were treated with CART at different doses(1,10 and 100 nmol/L) and at different time points.The expression of phosphorylated ERK(p-ERK) and BDNF were analyzed by Western blotting with or without PD98059,an ERK pathway inhibitor,treatment.RESULTS:CART increased expression of prolonged phosphorylated ERK in a time-and dose-dependent manner as compared with the controls(P0.01),and also increased expression of BDNF,which was inhibited by PD98059 pretreatment.CONCLUSION:BDNF synthesis induced by CART is mediated through ERK activation in hippocampal neurons." @default.
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- W2357282652 date "2007-01-01" @default.
- W2357282652 modified "2023-09-27" @default.
- W2357282652 title "Cocaine-and amphetamine-regulated transcript induces BDNF synthesis through ERK activation in hippocampal neurons" @default.
- W2357282652 hasPublicationYear "2007" @default.
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