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- W2357287362 abstract "The vitellogenin of male goldfish (]Carassius auratus) was induced by intraperitoneal injection of 0.05 mg·g~(-1)BW of 17β-estradiol dissolved in ethanol. After two weeks induction, the blood was collected from caudal vessel, then centrifuged at 8 000 r·min~(-1) for 5 min. After plasma native-PAGE, the gel was stained with the method of CBB G250-, phospho-, lipo- and glyco-staining respectively. the location of vitellogenin in the gel was identified. This method provides a practical and effective way of vitellogenin identification. The result of native-PAGE showed that, after the injection of 0.05 mg·g~(-1)BW of 17β-estradiol for two weeks, the male fish can synthesize vitellogenin. The vitellogenin average content in E_2 group was 690.2 ng·mL~(-1) by ELISA, it was significantly different from male control group(P0.01), whose average content was 10.7 ng·mL~(-1), and it was also much higher than that of the female control group, whose average content was 285.5 ng·mL~(-1). Compared with control male group, the Ca~(2+) and plasma total protein content of the E_2 exposed male group was increased significantly. In control group, the Ca~(2+) contents in plasma of male and female control groups were 1.6 mmol·L~(-1) and 1.7 mmol·L~(-1), respectively. After two-weeks treatment of E_2, Ca~(2+) content of male groups was 50% higher than that of male control group, which was 2.3 mmol·L~(-1). The contents of total protein in the control male and female groups were 3253.1 mg·L~(-1) and 7071.0 mg·L~(-1), respectively. After two-weeks treatment of E_2, the total protein content in plasma was 9616.6 mg·L~(-1), which is 3-folder higher than that of the control male group. So E_2 not only can induce male goldfish to synthesize vitellogenin but also can alter the physical index of fish plasma." @default.
- W2357287362 created "2016-06-24" @default.
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- W2357287362 date "2004-01-01" @default.
- W2357287362 modified "2023-09-25" @default.
- W2357287362 title "The identification of vitellogenin induction by 17β-estradiol in male Carassius auratus" @default.
- W2357287362 hasPublicationYear "2004" @default.
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