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- W2357304857 abstract "The objective of this study was to ascertain the species,distribution and genetic development of the endophytic fungus from the major locoweed in the west meadow of China and detect the antibacterial activity of their secondary metabolites.The study collected Oxytropis flabra,Oxytropis Ochrocephala Bunge,Oxytropis Kansuensis,Oxytropis Deflexa,Oxytropis Coerulea and Astragalus variabilis samples to isolate and purify the endophytic fungi in the different tissues,then used the morphology techniques and ITS sequence analysis to identify their species,and built the phylogenetic tree by Neighbor-Joining method.Staphylococcus aureus,Bacillus subtilis,Escherichia coli and Salmonella were selected as indicators to do the antibacterial tests of the fungi secondary metabolites by paper disc diffusion method.265 fungal colonies were isolated from the collected locoweed,which were combined to 38 kinds of different endophytic fungus.The distribution of the endophytic fungus in the tissues of locoweed was different;Fusarium sp.Alternaria sp.and Paecilomyces sp.were the dominant strains,which occupied 16.98%,11.33% and 9.81% of the total isolated colonies respectively.Phylogenetic analysis showed that 38 kinds of endophytic fungus could be divided into two populations based on the evolutionary kinship.The endophyte species and the distance of genetic relationship belonged to locoweed species,tissues,ecological environment and other factors.The antibacterial tests results showed that the secondary metabolites of 29 strains at least(76.32% of the total strains isolated) presented the antibacterial activity against one of the tested bacteria,of which the secondary metabolites of two strains were found to have stronger antibacterial activity against the four tested bacteria.There are various species of endophytic fungus in the major locoweed in the west meadow of China,the endophytes in different ecological environment have a certain kinship but also with some differences.Diverse antibacterial natural products are existed in the secondary metabolites of endophytic fungus from locoweed,which could be a new resource to screen active substance with antibacterial activity." @default.
- W2357304857 created "2016-06-24" @default.
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- W2357304857 date "2012-01-01" @default.
- W2357304857 modified "2023-09-23" @default.
- W2357304857 title "Study on Isolation,Identification and the Antibacterial Activity of Endophytic Fungi from the Major Locoweed in China" @default.
- W2357304857 hasPublicationYear "2012" @default.
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