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- W2357305836 abstract "Objective:To clone a novel deltamethrin-resistance associated opsin gene from Culex pipiens pallens. Methods:The left and right gene-specific primers were designed on the basis of the nucleotide sequences of the 289 bp known from the combination of suppression substractive hybridization(SSH) and gene expression profiling by cDNA arrays and used in 5’ and 3’ RACE to amplify opsin cDNA ends. The templates of 5’ and 3’ RACE were Plasmid cDNA library and the second-strand cDNA fragment amplified by RT-PCR, respectively. The 5’ and 3’ cDNAs of opsin obtained were sequenced, spliced and made bioinformatics analysis using softwares. Results:The six full-length sequences of opsin gene were obtained, named NYD-OP1,OP2,OP3,OP4,OP5 and OP6 respectively. There was an open reading frame of 1 116 bp in the sequence of NYD-OP1-2 cDNA(GenBank/NCBI AY297441,AY297442) and 1 119 bp in that of NYD-OP3-6(Genebank/NCBI AY297443,AY297444,AY299337,AY299338) respectively. Conclusion:The cDNAs of opsin gene were successfully cloned from Culex pipiens pallens." @default.
- W2357305836 created "2016-06-24" @default.
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- W2357305836 date "2005-01-01" @default.
- W2357305836 modified "2023-09-24" @default.
- W2357305836 title "Cloning of a novel deltamethrin-resistance associated opsin gene from Culex pipiens pallens" @default.
- W2357305836 hasPublicationYear "2005" @default.
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