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- W2357306541 abstract "Objective To construct three truncated SCIRR 10 protein mammalian cells systems,and discuss the protein function sites of SCIRR 10.Methods Three truncated SCIRR 10 genes were amplified by PCR,and three kinds of recombinant eukaryotic expression vectors were constructed as follows:pcDNA3.1/Myc-His-SCIRR 10(1-145aa),pcDNA3.1/ Myc-His-SCIRR 10(1-90aa) and pcDNA3.1/Myc-His-SCIRR 10(1-70aa).Then they were transiently transfercted into COS-7 cells at the same time.After 48 h,the recombinant proteins were detected by Western blotting.Results The molecular weights of three truncated SCIRR 10 proteins were 18 800,12 800 and 10 700.Three SCIRR 10 truncated proteins were successfully expressed in COS-7 cells.β-tubulin protein had no change.Conclusion The three truncated SCIRR 10 protein mammalian cell systems are constructed successfully,and the proteins are obtained by this system." @default.
- W2357306541 created "2016-06-24" @default.
- W2357306541 creator A5008231346 @default.
- W2357306541 date "2011-01-01" @default.
- W2357306541 modified "2023-09-24" @default.
- W2357306541 title "Construction of three truncated SCIRR 10 protein eukaryotic expression vectors and their expressions in COS-7 cells" @default.
- W2357306541 hasPublicationYear "2011" @default.
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