FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2357323345> ?p ?o ?g. }

• W2357323345 abstract "Background: Interferon- has various biologic effects, including antiviral effect, antitumor proliferative effect, activation of macrophage and B lymphocyte, and increased expression of major histocompatibility complex. Especially, antitumor proliferative effect of interferon- has already been proved to be important in vivo as well as in vitro. And, clinical studies of interferon- have been tried in lung cancer patients. However, the mechanism of antitumor effect of interferon- has not yet been established despite of many hypotheses. Necrosis is a type of cell death which is well known to occur in the circumstances of severe stresses. In contrast, is another type of cell death which occurs in such biological circumstances as embryonic development, regression of organs, and self-tolerance of lymphocytes. And, apoptosis is an active process of cell death in which cells are dying with fragmentations of their cytoplasms and nuclei. And, in the process of apoptosis the DNAs of cells are cleaved between nucleosomes by unidentified endonuclease and therefore DNAs of apoptotic cells result in a typical electrophoresis pattern known as DNA ladder pattern. Recently it has been suggested that cytotoxic effect of interferon- occurs via apoptosis. To elucidate the mechanism of antitumor cytotoxic effect of interferon-, we microscopically observed a lung cancer cell line, A549 which was treated with interferon-. We observed A545 treated with interferon- was dying fragmented. And so, we performed this study to find out that the mechanism of antitumor cytotoxic effect of interferon- be apoptosis. Method: We treated A549, human lung cancer cell line with various concentration of interferon- and quantified its cytotoxic effect of various periods, 24 hours, 72 hours and, 120 hours by MTT(dimethylthiazolyl diphenyltetrazolium bromide) bioassay. Also, after we treated A549 with 100 units/mi of interferon- for 120 hours, we observed the pattern of cell death with inverted microscope and we extracted DNAs from the dead A549 cells and observed the pattern of 1.5% agarose gel electrophoresis with ethidium bromide staining. Result: 1) Cytotoxic effect of interferon- on A549: For the first 24 hours, threre was little cytotoxic effect and for between 24 hours and 72 hours, there was the beginning of cytotoxic effect and for 120 hours there was increased cytotoxic effect. 2) Pattern of A549 cell death by interferon-: We observed with inverted microscope that A549 cells were dying fragmented. 3) DNA ladder pattern of gel electrophoresis: We observed DNA ladder pattern of gel electrophoresis of extracted DNAs from dead A549 cells. Conclusion: We concluded that the mechanism of interferon-induced cytotoxicity on lung cancer cell line, A549 be via apoptosis." @default.
• W2357323345 created "2016-06-24" @default.
• W2357323345 creator A5008837813 @default.
• W2357323345 creator A5024663629 @default.
• W2357323345 creator A5029619036 @default.
• W2357323345 creator A5049211215 @default.
• W2357323345 creator A5062961388 @default.
• W2357323345 creator A5069645289 @default.
• W2357323345 date "1996-01-01" @default.
• W2357323345 modified "2023-09-27" @default.
• W2357323345 title "The Mechanism of Interferon-gamma Induced Cytotoxicity on the Lung Cancer Cell Line, A549" @default.
• W2357323345 doi "https://doi.org/10.4046/trd.1996.43.1.63" @default.
• W2357323345 hasPublicationYear "1996" @default.
• W2357323345 type Work @default.
• W2357323345 sameAs 2357323345 @default.
• W2357323345 citedByCount "0" @default.
• W2357323345 crossrefType "journal-article" @default.
• W2357323345 hasAuthorship W2357323345A5008837813 @default.
• W2357323345 hasAuthorship W2357323345A5024663629 @default.
• W2357323345 hasAuthorship W2357323345A5029619036 @default.
• W2357323345 hasAuthorship W2357323345A5049211215 @default.
• W2357323345 hasAuthorship W2357323345A5062961388 @default.
• W2357323345 hasAuthorship W2357323345A5069645289 @default.
• W2357323345 hasConcept C109316439 @default.
• W2357323345 hasConcept C154317977 @default.
• W2357323345 hasConcept C190283241 @default.
• W2357323345 hasConcept C202751555 @default.
• W2357323345 hasConcept C203014093 @default.
• W2357323345 hasConcept C2776178377 @default.
• W2357323345 hasConcept C31573885 @default.
• W2357323345 hasConcept C502942594 @default.
• W2357323345 hasConcept C54355233 @default.
• W2357323345 hasConcept C55493867 @default.
• W2357323345 hasConcept C71924100 @default.
• W2357323345 hasConcept C81729549 @default.
• W2357323345 hasConcept C81885089 @default.
• W2357323345 hasConcept C86803240 @default.
• W2357323345 hasConceptScore W2357323345C109316439 @default.
• W2357323345 hasConceptScore W2357323345C154317977 @default.
• W2357323345 hasConceptScore W2357323345C190283241 @default.
• W2357323345 hasConceptScore W2357323345C202751555 @default.
• W2357323345 hasConceptScore W2357323345C203014093 @default.
• W2357323345 hasConceptScore W2357323345C2776178377 @default.
• W2357323345 hasConceptScore W2357323345C31573885 @default.
• W2357323345 hasConceptScore W2357323345C502942594 @default.
• W2357323345 hasConceptScore W2357323345C54355233 @default.
• W2357323345 hasConceptScore W2357323345C55493867 @default.
• W2357323345 hasConceptScore W2357323345C71924100 @default.
• W2357323345 hasConceptScore W2357323345C81729549 @default.
• W2357323345 hasConceptScore W2357323345C81885089 @default.
• W2357323345 hasConceptScore W2357323345C86803240 @default.
• W2357323345 hasLocation W23573233451 @default.
• W2357323345 hasOpenAccess W2357323345 @default.
• W2357323345 hasPrimaryLocation W23573233451 @default.
• W2357323345 hasRelatedWork W1527490033 @default.
• W2357323345 hasRelatedWork W1846681432 @default.
• W2357323345 hasRelatedWork W1896734497 @default.
• W2357323345 hasRelatedWork W1994174524 @default.
• W2357323345 hasRelatedWork W2053626724 @default.
• W2357323345 hasRelatedWork W2054764182 @default.
• W2357323345 hasRelatedWork W2078200326 @default.
• W2357323345 hasRelatedWork W2079838472 @default.
• W2357323345 hasRelatedWork W2086786445 @default.
• W2357323345 hasRelatedWork W2165289449 @default.
• W2357323345 hasRelatedWork W2181518859 @default.
• W2357323345 hasRelatedWork W2350641186 @default.
• W2357323345 hasRelatedWork W2352091299 @default.
• W2357323345 hasRelatedWork W2373118421 @default.
• W2357323345 hasRelatedWork W2383321349 @default.
• W2357323345 hasRelatedWork W2392287060 @default.
• W2357323345 hasRelatedWork W2411381753 @default.
• W2357323345 hasRelatedWork W2412577704 @default.
• W2357323345 hasRelatedWork W2416666332 @default.
• W2357323345 hasRelatedWork W2996028972 @default.
• W2357323345 isParatext "false" @default.
• W2357323345 isRetracted "false" @default.
• W2357323345 magId "2357323345" @default.
• W2357323345 workType "article" @default.