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- W2357339731 abstract "Objective:To investigate the effect of Jie gu san Containing Serum on the proliferation and osteogenic differentiation of rat bone mesenchymal stem cells.Methods:Rats were giving Jie gu san by high,moderate and low intensity respectivrly and their serum were obtained after 7 days.The control serum were obtained by giving equal volume physiological saline.Rat bone mesenchymal stem cells(rBMSCs) were obtained from Wistar rats and screened by the adhesive method.The serum were supplemented into the culture medium of rat mesenchymal stem cells by different concentration.The proliferation of rBMSCs was analyzed by MTT reduction assay.The osteogenic differentiation markers including ALP activity,CFU-FALP,calcium deposition and mineralized bone modulus were compared among the exposed groups and the control.Results:Moderate Jie gu san containing serum shows a strong stimulation of cell proliferation and significantly enhances the osteogenic differentiation of rMSCs,indicated by significantly improved ALP activity,calcium deposition,CFU-FALP and the number of mineralized bone nodules compared to the control and other groups.Conclusion:Jie gu san Containing Serum can strongly stimulate the proliferation and promote their osteogenesis differentiation of rat mesenchymal stem cells." @default.
- W2357339731 created "2016-06-24" @default.
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- W2357339731 date "2011-01-01" @default.
- W2357339731 modified "2023-09-23" @default.
- W2357339731 title "Effects of Jie gu san Containing Serum on the Proliferation and Osteogenesis Differentiation of Rat Mesenchymal Stem Cells" @default.
- W2357339731 hasPublicationYear "2011" @default.
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