Matches in SemOpenAlex for { <https://semopenalex.org/work/W2357365131> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2357365131 abstract "Objective:To construct the recombinant adenovirus vector carrying rat serum amyloid P component(SAP)as preparation for later use.Methods:Rat SAP was obtained by using RT-PCR amplification and then cloned into the shutter plasmid pAdTrace-TO4.Subsequently,this newly constructed plasmid pAdTrace-TO4-SAP was followed by homologous recombination with bone plasmid pAdEasy-1 in BJ5183.Recombinant adenovirus was obtained after being packaged in human embryonic kidney cells HEK293 by lipofectAmineTM2000 mediation.After amplification the recombined adenovirus,we determined its titer by end-point dilution assay.Results:In recombinant shuttle plasmid and recombinant adenovirus,a fragment of 700 bp detected by PCR was identical with that included in Genbank.The titer of recombinant adenovirus reached 2.8×108 pfu/ml.Conclusion:The recombinant adenovirus containing SAP gene was constructed successfully.This will provides a good basis for further study about the influence of SAP on lung fibrosis." @default.
- W2357365131 created "2016-06-24" @default.
- W2357365131 creator A5018136969 @default.
- W2357365131 date "2010-01-01" @default.
- W2357365131 modified "2023-09-28" @default.
- W2357365131 title "Cloning of rat serum amyloid P component gene and preparation of its recombinant adenovirus vector" @default.
- W2357365131 hasPublicationYear "2010" @default.
- W2357365131 type Work @default.
- W2357365131 sameAs 2357365131 @default.
- W2357365131 citedByCount "0" @default.
- W2357365131 crossrefType "journal-article" @default.
- W2357365131 hasAuthorship W2357365131A5018136969 @default.
- W2357365131 hasConcept C102744134 @default.
- W2357365131 hasConcept C104317684 @default.
- W2357365131 hasConcept C121050878 @default.
- W2357365131 hasConcept C145085861 @default.
- W2357365131 hasConcept C145640855 @default.
- W2357365131 hasConcept C153911025 @default.
- W2357365131 hasConcept C159047783 @default.
- W2357365131 hasConcept C174510640 @default.
- W2357365131 hasConcept C199360897 @default.
- W2357365131 hasConcept C22744801 @default.
- W2357365131 hasConcept C2522874641 @default.
- W2357365131 hasConcept C2776954459 @default.
- W2357365131 hasConcept C32470452 @default.
- W2357365131 hasConcept C32611913 @default.
- W2357365131 hasConcept C40767141 @default.
- W2357365131 hasConcept C41008148 @default.
- W2357365131 hasConcept C54355233 @default.
- W2357365131 hasConcept C86803240 @default.
- W2357365131 hasConcept C92087593 @default.
- W2357365131 hasConceptScore W2357365131C102744134 @default.
- W2357365131 hasConceptScore W2357365131C104317684 @default.
- W2357365131 hasConceptScore W2357365131C121050878 @default.
- W2357365131 hasConceptScore W2357365131C145085861 @default.
- W2357365131 hasConceptScore W2357365131C145640855 @default.
- W2357365131 hasConceptScore W2357365131C153911025 @default.
- W2357365131 hasConceptScore W2357365131C159047783 @default.
- W2357365131 hasConceptScore W2357365131C174510640 @default.
- W2357365131 hasConceptScore W2357365131C199360897 @default.
- W2357365131 hasConceptScore W2357365131C22744801 @default.
- W2357365131 hasConceptScore W2357365131C2522874641 @default.
- W2357365131 hasConceptScore W2357365131C2776954459 @default.
- W2357365131 hasConceptScore W2357365131C32470452 @default.
- W2357365131 hasConceptScore W2357365131C32611913 @default.
- W2357365131 hasConceptScore W2357365131C40767141 @default.
- W2357365131 hasConceptScore W2357365131C41008148 @default.
- W2357365131 hasConceptScore W2357365131C54355233 @default.
- W2357365131 hasConceptScore W2357365131C86803240 @default.
- W2357365131 hasConceptScore W2357365131C92087593 @default.
- W2357365131 hasLocation W23573651311 @default.
- W2357365131 hasOpenAccess W2357365131 @default.
- W2357365131 hasPrimaryLocation W23573651311 @default.
- W2357365131 hasRelatedWork W2347487394 @default.
- W2357365131 hasRelatedWork W2348822029 @default.
- W2357365131 hasRelatedWork W2349749838 @default.
- W2357365131 hasRelatedWork W2351742304 @default.
- W2357365131 hasRelatedWork W2361262298 @default.
- W2357365131 hasRelatedWork W2366414864 @default.
- W2357365131 hasRelatedWork W2371503002 @default.
- W2357365131 hasRelatedWork W2374413524 @default.
- W2357365131 hasRelatedWork W2377320522 @default.
- W2357365131 hasRelatedWork W2378373788 @default.
- W2357365131 hasRelatedWork W2379156367 @default.
- W2357365131 hasRelatedWork W2379517162 @default.
- W2357365131 hasRelatedWork W2379535871 @default.
- W2357365131 hasRelatedWork W2379632582 @default.
- W2357365131 hasRelatedWork W2386176247 @default.
- W2357365131 hasRelatedWork W2392670202 @default.
- W2357365131 hasRelatedWork W2392697370 @default.
- W2357365131 hasRelatedWork W2415789677 @default.
- W2357365131 hasRelatedWork W2422596936 @default.
- W2357365131 hasRelatedWork W3029690152 @default.
- W2357365131 isParatext "false" @default.
- W2357365131 isRetracted "false" @default.
- W2357365131 magId "2357365131" @default.
- W2357365131 workType "article" @default.