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- W2357366178 abstract "In order to study the genomic structure and functional of Siniperca chuatsi lipoprotein lipase(LPL) and hepatic lipase(HL) genes,at first,two full-length cDNA clones of LPL and HL were isolated from the liver of Siniperca chuatsi by RT-PCR and RACE. The LPL cDNA was 2089 bp in length,and contains a 1548 bp open reading frame (ORF) encoding 515 amino acids. The HL cDNA was 1 964 bp in length,and contains a 1 494 bp ORF encoding 497 amino acids. Phylogenetic analysis showed that Siniperca chuatsi LPL and other vertebrate LPL are grouped in one cluster,as well as all the HL sequences grouped in the other cluster. LPL and HL genomic sequences were identified in the Siniperca chuatsi genome and displayed the same genomic structure as other vertebrates(LPL:10 exons and 9 introns;HL:9 exons and 8 introns) spanning 7.4 kb and 8.8 kb,respectively. Using genome walker method,the LPL and HL 5'-flanking regions were obtained in the Siniperca chuatsi which spanned 1071 bp and 2173 bp,respectively, and several potential regulatory elements were identified in the promoter regions. The mRNA expression of Siniperca chuatsi LPL gene was detected in all the examined tissues:highest in adipose tissue,followed by liver,brain,intestine, muscle,and lowest in spleen. But the Siniperca chuatsi HL gene was found to be exclusively expressed only in liver. These studies will lay a foundation in the regulation of gene expression in lipid metabolism and the prevention from the development of atherosclerosis." @default.
- W2357366178 created "2016-06-24" @default.
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- W2357366178 date "2009-01-01" @default.
- W2357366178 modified "2023-09-23" @default.
- W2357366178 title "Genomic structure and expression of lipoprotein lipase(LPL) and hepatic lipase(HL) genes in Siniperca chuatsi" @default.
- W2357366178 hasPublicationYear "2009" @default.
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