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- W2357367077 abstract "The coding sequence of a single chain antibody (2a10) against circumsporozoite protein (CSP) of Plasmodium falciparum was modified according to Anopheles gambiae preferred codons. Totally, 170 nucleotides in six kinds of amino acids were replaced and then linked with the coding sequence from A.gambiae cecropin A. Subsequently, the fusion gene, cecrop-m2a10, was subcloned into the expression vector pET32a(+)and expressed in E.coli BL21(DE3). Expression products were analyzed by SDS-PAGE, Western-blot and Agar-diffusion method. Results indicated that the cecrop-m2a10 fusion gene was correctly cloned into expression vector pET32a(+)and was highly expressed in E.coli as an insoluble form. After the collection of inclusion body, denaturing lysis and refoding dialysis, the purity of the expression product was over 70% and showed bacteriostasis inhibition to the growth of E.coli DH5α. Our experiment provided significant data for further study on the multiple anti-pathogen effect of cecrop-m2a10 gene in transgenic mosqitoes." @default.
- W2357367077 created "2016-06-24" @default.
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- W2357367077 date "2008-01-01" @default.
- W2357367077 modified "2023-09-24" @default.
- W2357367077 title "CONSTRUCTION, PROKARYOTIC EXPRESSION AND PRELIMINARY IDENTIFICATION OF THE BIOACTIVITY OF THE FUSION GENE M2A10 AND CECROPIN A" @default.
- W2357367077 hasPublicationYear "2008" @default.
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