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- W2357372252 abstract "Analysis of metabolic flux distribution in fermentation is favorable to understanding the efficiency of the process. In this study, glycerol fermentation was carried out in a computer-controlled 15L-fermentor, and on-line data including oxygen and carbon dioxide contents in the exit gas were collected in the computer. A complex medium containing glucose, corn steep liquor, and urea was used. Ethanol was the main by-product during the cell growth phase, while gluconate and pyruvate were accumulated during the glycerol production phase. Carbon balance revealed that more than 96% of the carbon in the medium was recovered in the cell and measured metabolites, and the contribution of both corn steep liquor and glucose to cell growth was considered. According to the specific rates of cell growth, glucose consumption and metabolites formation, the metabolic flux distribution was estimated by 17 stoichiometrical equations. Although the specific carbon dioxide evolution rate was not used as a constraint, the calculated specific evolution rate was consistent with the measured one. During the typical growth phase (6~12h), carbon fluxes to monomers of the cellular structural macromolecules and that to ethanol were 53.8% and 11.9% respectively based on the flux of glucose uptake, while that to glycerol was only 2.1%. During the typical production phase (48~54h), the cells stopped growing, and the carbon flux to glycerol was increased to 42% and those to organic acids accounted for 11%. These results suggested the ways to improve glycerol fermentation, that is, suppression of ethanol formation during the growth phase and limitation of the formation of fatty acids during the production phase." @default.
- W2357372252 created "2016-06-24" @default.
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- W2357372252 date "2002-01-01" @default.
- W2357372252 modified "2023-09-25" @default.
- W2357372252 title "Metabolic Flux Distribution in Batch Cultivation of Candida krusei for Glycerol Production" @default.
- W2357372252 hasPublicationYear "2002" @default.
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