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- W2357376792 abstract "OBJECTIVE:To establish the method for the simultaneous determination of tretinoin and chlorpheniramine maleate in compound tretinoin ointment.METHODS:HPLC method was adopted.Luna C18 column was used with mobile phase consisted of methanol-phosphate buffer(0.02 mol·L-1 potassium dihydrogen phosphate,pH adjusted to 7.0 using 1 mol·L-1 sodium hydroxide,V/V=80:20) at a flow rate of 1.0 mL·min-1.The column temperature was 30 ℃ and injection volume was 20 μL.The detection wavelength was set at 350 nm and 262 nm for tretinoin and chlorpheniramine maleate,respectively.The content of tretinoin and chlorpheniramine maleate was determined by external standard method.RESULTS:The liner range of tretinoin was 20~100 μg·mL-1(r=0.999 9) with an average recovery rates of it at low,medium and high concentrations were 98.48%,97.15%,98.88%(RSD=0.92%).The liner range of chlorpheniramine maleate was 30~150 μg·mL-1(r=0.999 9) with an average recovery rates of it at low,medium and high concentrations were 100.47%,99.01%,99.75%(RSD=0.83%).CONCLUSION:The established method is simple,accurate and reproducible.It can be used for the quality control of compound tretinoin ointment." @default.
- W2357376792 created "2016-06-24" @default.
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- W2357376792 date "2012-01-01" @default.
- W2357376792 modified "2023-09-24" @default.
- W2357376792 title "Simultaneous Content Determination of 2 Components in Compound Tretinoin Ointment by Dual Wavelength HPLC" @default.
- W2357376792 hasPublicationYear "2012" @default.
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