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- W2357394501 abstract "Objective To investigate whether vascular endothelial growth factor (VEGF) gene plasmid earryiing polytetrafluoroethylene (PTFE) vascular graft material could transfect endothelial cells (EC) and promote ECs growth.Methods The PTFE vascular graft materialscarrying pCDI-hVEGF_(121), pCDI or pEGFP plasmids were soaked in saline and the values of A2.60nm at different time points were measured,then the controlled release curve was made.ECs were derived from human umbilical vein and seeded on the PTFE materials carrying pCDI-hVEGF_(121),pCDI or pEGFP.The number of ECs was counted and VEGF protein concentrations were measured by ELISA method at different time points.The GFP expression in ECs on the PTFE materials carrying pEGFP was examined under the fluorescence mi- croscopy.Results The controlled release curve showed the gene was released quickly during 0.5-4.0 h, then became slow,and released continuously even after 72 h.At 24,72 and 120 h,the number of ECs and proliferation rate on PTFE materials carrying pCDI-hVEGF_(121) were higher than that of PTFE materials carrying pCDI or pEGFP (P＜0.05).The VEGF protein concentration in PTFE materials carrying pC- DI-hVEGF_(121) was higher than that of PTFE materials carrying pCDI or pEGFP at 6,24,72 and 120 h (P＜0.01).The GFP expression in ECs on the PTFE materials carrying pEGFP could be detected under the fluorescence microscopy.Conclusion The PTFE graft can be used as carrier of VEGF gene plasmid. VEGF gene can transfect ECs and promote ECs growth." @default.
- W2357394501 created "2016-06-24" @default.
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- W2357394501 date "2006-01-01" @default.
- W2357394501 modified "2023-09-26" @default.
- W2357394501 title "Endothelial cell proliferation on polytetrafluoroethylene vascular graft materials containing VEGF gene plasmid" @default.
- W2357394501 hasPublicationYear "2006" @default.
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