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- W2357399938 abstract "Objective:To establish the high sensitive method for sabutamol and clenbuterol in animal-based food.Methods:The qualitative ELISA was based on the specific immuno-chemical reactions between antigen and antibody.ELISA was used for screening sabutamol and clenbuterol in animal-based food and the positive results were proved with HPLC-MS/MS.Results:The linear range of the ELISA method was 0.06～4.0 μg/L,and the correlation coefficient of the standard curve-0.996～-0.999.The recoveries of sabutamol for the spiked animal-based food ranged from 71.2% to 87.2% and of clenbuterol ranged from 70.4% to 85.2% with an average recovery of 81.7% and 80.1%.The linear range of the HPLC-MS/MS method was 0.1～10.0 μg/L with linear correlation coefficient of 0.9998 for sabutamol and of 0.9992 for clenbuterol.The limit of detection was 0.2 and 0.5 μg/kg.The recoveries of sabutamol for the spiked samples ranged from 89.9% to 96.7% and those of clenbuterol ranged from 88.4% to 96.2%.Conclusion:The ELISA method is sensitive with simple sample extracting,and suitable for detection for trace sabutamol and clenbuterol of mounts of sample with lower cost.The HPLC-MS/MS method is suitable for sabutamol and clenbuterol detection and structure proof with high sensitivity and anti-interference." @default.
- W2357399938 created "2016-06-24" @default.
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- W2357399938 date "2009-01-01" @default.
- W2357399938 modified "2023-09-23" @default.
- W2357399938 title "Research of qualitative ELISA and HPLC-MS/MS for sabutamol and clenbuterol in animal-based food" @default.
- W2357399938 hasPublicationYear "2009" @default.
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