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- W2357441532 abstract "Objective To observe the expression of survivin protein on the gastric cancer cell line SGC-7901 and the effect of different concentration antisense oligonucleotide(ASODN) targeting survivin on the growth of the gastric cancer cell line SGC-7901.Methods Cellular immunohistochemistry was used to observe the expression of survivin protein in the gastric cancer cell line SGC-7901.The cultured cells were divided into 8 groups:control group,lipofectin group,200 nmol/L none sense oligonucleotide(NSODN) group,400 nmol/L NSODN group,600 nmol/L NSODN group,200 nmol/L ASODN group,400 nmol/L ASODN group,600 nmol/L ASODN group.The rate of cell growth inhibition(IR) of all groups were detected by MTT colorimetry after transfected 48 hours.The rate of cells apoptosis with 600 nmol/L NSODN group and 600 nmol/L ASODN group and the expression of survivin protein were detected by flow cytometry on the gastric cancer cell line SGC-7901 quantitively.Results Survivin protein was expressed in karyon and cytoplasm,mainly expressed in karyon.After transfected for 48 hours,the IR of ASODN groups were significantly higher than control group,lipofectin group and oligonucleotide groups(P0.01).The intensity of inhibition depended with concentration.The inhibiting rate of 600 nmol/L groups reached 40.0%.Flow cytometry demonstrated that ASODN targeting survivin induce apoptosis,survivin protein was 8.8% of control groups(P0.01).Conclusion ASODN targeting survivin with different concentration can inhibit the growth of the gastric cancer cell line SGC-7901 by decreasing the expression of survivin gene protein." @default.
- W2357441532 created "2016-06-24" @default.
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- W2357441532 date "2005-01-01" @default.
- W2357441532 modified "2023-09-24" @default.
- W2357441532 title "The Effect of Antisense Oligonucleotide Targeting Survivin on the Growth of the Gastric Cancer Cell Line SGC-7901" @default.
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