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- W2357444868 abstract "ObjectiveTo select proper passages of human preadipocyte for seeds cells of adpose tissue engineering.MethodsPreadipocytes were isolated from subcutaneous adipose tissue of voluntary adults who needed an operation to remove ilium for bone grafting.The cells were proliferated and passaged till 18 passages.Preadipocytes of 1-18 passages were selected and the senescence was assessed through senescent associated β-galatidase staining (SA-β-Gal) and morphological observation under phase reverse microscopy.ResultsCells within 10 passages were proliferated rapidly,those from 10 to 15 passages grew slowly gradually and those beyond 15 passages showed significant senescence.The SA-β-Gal postive rate of preadipocytes within 15 passages had no significant difference ( P 0.05),while that of pradipocytes from 15 to 18 passages was obviously increased.ConclusionPreadipocytes beyond 15 passages showed a low proliferative capability and significant senescence,and are not suitable for seeds cells for adipose tissue engineering." @default.
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- W2357444868 date "2004-01-01" @default.
- W2357444868 modified "2023-09-25" @default.
- W2357444868 title "Diverse senescence of different passages of human preadipocyte cultured in vitro" @default.
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