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- W2357474457 abstract "Objective To culture dendritic cells(DC) in vitro and to induce specific anti-tumor immunoreaction in cancer cell lines with superantigen HAS and DC. Methods DC was cultured from PBMC by using GM-CSF and IL-4. Lymphocytes stimulated by HAS were named HASL. DC and lymphocyte or HASL were cocultured to induce specific anti-tumor effect, and the obtained cells were named DC-L or DC-HASL. Phenotypes of DC and HASL were analysed by FCS. MTT assay was used to determine cytotoxicity in vitro. The anti-tumor effects on GLC-82 bearing mice were also observed. Results DC-HASL could lyse GLC-82 cell specifically in vitro , but HASL and DC-L had no obvious specificity. These three effector cells can all inhibit the growth of tumor in nude mice, and the most effective one was DC-HASL. Conclusion High potency and relatively specific anti-tumor immunocyte can be prepared in virtue of DC and HAS." @default.
- W2357474457 created "2016-06-24" @default.
- W2357474457 creator A5076516883 @default.
- W2357474457 date "2002-01-01" @default.
- W2357474457 modified "2023-09-23" @default.
- W2357474457 title "High potency and specific anti-tumor immunoreaction induced by dendritic cells and superantigen" @default.
- W2357474457 hasPublicationYear "2002" @default.
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