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- W2357496986 abstract "Ochratoxin A(OTA) mimotope was displayed on phage surface.Two complementary oligonucleotides which contained OTA mimotope DNA sequences were cloned into vector pC89S4.Helper phage MOI(multiplicity of infection) and IPTG addition were optimized to gain phages with high density expression of OTA mimotope through phage display technology.The expression efficiency was measured by ELISA analysis.The result showed that high density OTA mimotope phages were obtained successfully.Helper phage MOI and IPTG concentration showed little influence the expression of OTA mimotope.However,IPTG addition at different time points had great effects on the expression of OTA mimotope.The expression efficiency was greatly improved after optimization." @default.
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- W2357496986 date "2012-01-01" @default.
- W2357496986 modified "2023-09-27" @default.
- W2357496986 title "High Density Expression of Ochratoxin A Mimotope Based on Phage Display Technology" @default.
- W2357496986 hasPublicationYear "2012" @default.
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