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- W2357505587 abstract "Objective: To develop an effective method to detect circulating micrometastases in patients with pancreatic cancer. Methods: Peripheral blood mononuclear cells(PBMC) from normal subject were mixed with human pancreatic cancer PANC-1 cells in different proportions.The mixed cells of each proportion were sorted by using EpCAM-antibody-coated immunomagnetic nanobeads for separation and enrichment of the tumor cells.Then,the expression of human telomerase reverse transcriptase(h-TERT) and c-met in the tumor cells were detected by RT-nest-PCR,so as to determine the sensitivity of this method.The expressions of h-TERT and c-met in the PBMC from 25 patients with pancreatic cancer and 15 patients with benign diseases were detected by using the above combined immunomagnetic nanobeads and RT-nest-PCR method.The relations of both genes with the clinicopathologic factors were also analyzed. Results: The sensitivity of the combined immunomagnetic nanobeads and RT-nest-PCR method for h-TERT and c-met was 1 tumor cell/1×107 PBMC and 1 tumor cell/1×106 PBMC,respectively.The expression rates of h-TERT and c-met both were 0(0/15) in benign patients,and was 100%(25/25) and 80%(20/25) in pancreatic cancer patients,respectively.Moreover,the positive expression rate of c-met was significantly related to the tumor stage(P0.05). Conclusion: Detection of h-TERT and c-met using combined immunomagnetic nanobeads and RT-nest-PCR is a highly sensitive method for identifying the micrometastases of pancreatic cancer in peripheral circulation." @default.
- W2357505587 created "2016-06-24" @default.
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- W2357505587 date "2012-01-01" @default.
- W2357505587 modified "2023-09-23" @default.
- W2357505587 title "Combination of immunomagnetic nanobeads and RT-nest-PCR for detection of micrometastases of pancreatic cancer in peripheral circulation" @default.
- W2357505587 hasPublicationYear "2012" @default.
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