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- W2357517727 abstract "Objective To genotype the plasmid DNA of 34 clinical Escherichia coli and Klebsiella pneumoniae isolates producing blaCTX-M by denaturing high-performance liquid chromatography(DHPLC) and evaluate its sensitivity and speciality,and explore a rapid and convenient method for detecting the genotypes of CTX-M-type extended-spectrum beta-lactamases(blaCTX-M).And investigating the antibiotic resistance of blaCTX-M producing clinical isolates of Escherichia coli and Klebsiella pneumonia.Methods Plasmid DNA from each blaCTX-M producing strain was subjected to PCR amplification.We used DHPLC to analyze the PCR production,and then DNA sequencing was performed to determine the genotypes.Finally we compared their results.And the drug susceptibility was performed by Kirby-Bauer disk diffusion.Results Thirty-five amplicons were amplified from 34 isolates,one of them producing two blaCTX-M.By analyzing the results of DHPLC and DNA sequencing,each peak pattern was corresponded to specific genotype.And the blaCTX-M producing strains were hypersensitive to imipenem and meropenem while had high resistance to ampicillin,cefazolin,cefotaxime,ceftriaxone,cefuroxime-sodium,piperacillin.Conclusion DHPLC not only has high accuratissime,but also is a convenient and rapid technique for detecting genotypes.So we can use DHPLC to detect the genotypes of blaCTX-M.BlaCTX-M producing strains expressed multiple drug resistance phenotypes.Imipenem or Meropenem is the best drug in the treatment of infections caused by blaCTX-M producing strains." @default.
- W2357517727 created "2016-06-24" @default.
- W2357517727 creator A5026734165 @default.
- W2357517727 date "2010-01-01" @default.
- W2357517727 modified "2023-09-25" @default.
- W2357517727 title "Detection of the genotypes of bla_(CTX-M) and antibiotic resistance in extended-spectrum beta-lactamases producing clinical isolates of Escherichia coli and Klebsiella pneumonia" @default.
- W2357517727 hasPublicationYear "2010" @default.
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