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- W2357578356 abstract "Objective To evaluate reliability of the cloned homogeneous immunoassay technique for detection of P53 protein content in serum and stool from patients with gastric carcinoma. Methods To prepare EA, ED and ED P53 fusion protein of beta galactosidase by PGEX 4T 2 expression vector, a novel cloned homegeneous immunoassay was establishment for detection of P53 protein in serum and stool samples from patients with gastric carcinoma and non gastric carcinoma groups as well as healthy control. Results The concentration of P53 protein in 31 gastric carcinoma subjects were remarkably increased as compared with 56 non gastric carcinoma ( P 0 01). Further studies showed that all cases were in accordance with immunohistochemical staining. Moreover, there existed P53 IgG immuno compound in these patients, these results implied that the entity type of P53 might contribute to prolong its halftime in peripheral circulation. There was preferential relation between cloned homegeneous immunoassay and ELISA in 10 samples. Conclusion Serum and stool P53 protein might be an independent biological marker in diagnosis of gastric carcinoma." @default.
- W2357578356 created "2016-06-24" @default.
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- W2357578356 date "2004-01-01" @default.
- W2357578356 modified "2023-09-25" @default.
- W2357578356 title "Establishment of cloned homogeneous immunoassay for detection of P53 protein in serum and stool samples from patients with gastric carcinoma" @default.
- W2357578356 hasPublicationYear "2004" @default.
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