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- W2357608575 abstract "Objective To study and analyze the differences among the strains in the genus Burkholderia at gene levels by comparing 16S～23S rRNA gene intergenic spacer region(ISR) sequences of Pseudomonas cocovenenans subsp farinofermentans strains isolated in Chinese foods and Burkholderia gladioli model strains.The phylogenetic relationships of them were expounded.Method Two pairs of specific primers for the genus Burkholderia were designed and 16S～23S rRNA gene ISR sequences were amplified for the strains.16-23S rRNA gene ISR sequences of 3 international reference strains representative of the three different pathogenic strains of Burkholderia gladioli(ATCC10248,NCPPB 947,NCPPB3580),and 1 strain of B.phenazinium(LMG2247)and 8 strains of Pseudomonas cocovenenans subsp farinofermentans isolates(HN2y、Co14、Co8、Co36、Sx8801、90-3、56(2)、 56(2))from the different samples of food-poisoning in China were determined.The total sequences were compared and analyzed with the relative strain sequences in the GenBank by the molecular biological analyzing software.Results Two highly variable ISR regions and the DNA specific sequences associated with isolate strains produced the Bongkrekic acid were found.The results of the DNA sequences were submitted to the GenBank.The phylogenetic tree of the isolates and the Burkholderi gladioli were illustrated.Conclusion New and reliable theory and technology supports were provided for the further study of the identification of Pseudomonas cocovenenans subsp farinofermentans isolates produced Bongkrekic acid,research into their mechanisms in molecular levels and their phylogenetic relationships." @default.
- W2357608575 created "2016-06-24" @default.
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- W2357608575 date "2008-01-01" @default.
- W2357608575 modified "2023-09-24" @default.
- W2357608575 title "Study on Comparison of 16S～23S rRNA Gene ISR Sequence of Pseudomonas cocovenenans subsp farinofermentans Strains and Burkholderia gladioli Strains" @default.
- W2357608575 hasPublicationYear "2008" @default.
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