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- W2357609512 abstract "Objective: To study the value of the measurement of Dermatophagoides pteronyssinus-specific IgE concentration in sera in the diagnosis of perennial allergic rhinitis using ELISA. Methods: In this study we evaluated the performance of a Dermatophagoides pteronyssinus-specific IgE method, developed by indirect ELISA. A panel of 30 positive control sera and 35 clinical sera were tested for Der P specific IgE via UniCAP system and our quantitative ELISA kit. Results: Statistical analysis revealed that there was no significant difference between the two methods. The sensitivity of our ELISA system was 0. 37 IU/ml, and precision levels were 7.5%, 5.7%, 5.9% for in-traassay and 12. 3% , 12. 7%, 9. 4% for interassay at the concentration of 2 lU/ml, 14. 3 lU/ml, 24. 7 lU/ml, respectively. Conclusion: Our indirect ELISA methodology determining Der P specific IgE in sera compares favorably with the results obtained by the UniCAP system. The indirect ELISA method may contribute to the diagnosis of perennial allergic rhinitis." @default.
- W2357609512 created "2016-06-24" @default.
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- W2357609512 date "2005-01-01" @default.
- W2357609512 modified "2023-09-24" @default.
- W2357609512 title "Analysis of Dermatophagoides Pteronyssinus-specific IgE Concentration in Sera of Perennial Allergic Rhinitis Using Indirect ELISA" @default.
- W2357609512 hasPublicationYear "2005" @default.
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