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- W2357629709 abstract "Aim To investigate the mechanism of electroacupuncture(EA) effects on promoting revascularization in SD rats brain of focal cerebral ischemia/reperfusion by discussing the expression of placental growth factor(PLGF)/Flt-1 pathway after middle cerebral artery occlusion(MCAO). Methods The SD rats received filament occlusion of the right middle cerebral artery for 2 h.SD rats were randomly divided into control group,model group and EA group.The modle group and EA group were divided into three subgroups according to accepting reperfusion 1 d,3 d,7 d after 2 h ischemia.After 1 h of the reperfusion,EA was bilateral Hegupoint in the EA group.Immunohistochemical method was used to detect the expression of PLGF and Flt-1 protein in the cortical ischemic region.RT-PCR was used to detect the expressiom of PLGF and Flt-1 mRNA.Western blotting was employed to detect the expression of PLGF protein. Results Compared with the control group,the mRNA and protein expression of PLGF and Flt-1 in the cortical ischemic region of the model group and EA group were significantly increased(P0.05).Compared with the model group,the mRNA and protein expression of PLGF and Flt-1 in the EA group were significantly increased(P0.05). Conclusion EA may upregulate expression of PLGF,Flt-1 protein and PLGF,Flt-1 mRNA in the cortical ischemic region,and PLGF/Flt-1 may promote revascularization in the rats brain of focal cerebral ischemia/reperfusion." @default.
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- W2357629709 date "2010-01-01" @default.
- W2357629709 modified "2023-09-25" @default.
- W2357629709 title "The Effects of Electroacupuncture on mRNA and Protein Expression of Placental Growth Factor and Flt-1 and Promotes Revascularization in the Brain After Focal Cerebral Ischemia/Reperfusion in SD Rats" @default.
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