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- W2357632917 abstract "Objective To establish method for the determination of 7 free fatty acids in human serum.Methods By adding n-Heptadecanoic acid as internal standard,Stearic Acid,oleic acid,linoleic acid,Linolenic acid, arachidonic acid,eicosapentaenoic acid and Docosahexaenoic Acid in human serum were methylesterified by H_2SO_4/methanol,extracted and concentrated by n-hexane,detected by gas chromatography.Results 7 kinds of fatty methyl ester could be separated completely after methylesterified,The linearity were ranged from 5.0～500μg/mL(r0.999),Minimum Limited Determination≤1.1μg/mL,The average recovery rate of 3 levels (20μg/mL,100μg/mL,300μg/mL) was91.7%～98.1%,RSD 1.9%～4.1%.Conclusion The method could be used to the determination of free fatty acids in human serum." @default.
- W2357632917 created "2016-06-24" @default.
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- W2357632917 date "2010-01-01" @default.
- W2357632917 modified "2023-09-23" @default.
- W2357632917 title "Determination Method Rearch of 7 Kinds Polyunsaturated Fatty Acid in Human Serum" @default.
- W2357632917 hasPublicationYear "2010" @default.
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