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- W2357646957 abstract "Purpose To investigate the preparation methods of TEM samples for cultured human epidermal melanocytes and to observe the ultrastructures of the cells.Methods Melanocytes were isolated from children's foreskins and cultured in M254 medium that was supplemented with human melanocyte growth supplement.Purified melanocytes were harvested from culture flask and TEM sample was prepared by conventional method,or cultured on PET film and allowed to attach and then TEM sample was prepared,respectively.Results Transmission electron microscopy showed that the ultrastructure of melanocytes and dendrites in transverse section were observed in samples prepared with conventional method.The intact ultrastructure of melanocytes and their dendrites could be observed in longitudinal section while PET film was applied as cellular support.Conclusions The PET film is an excellent cellular supporting material for the cultivation of human epidermal melanocytes.The ultrastructures of human epidermal melanocytes could be observed in samples prepared in different ways.But the sample that using PET film as cellular support is much beneficial for the ultrastructural observation of metabolism and transferring of melanosome in melanocytes." @default.
- W2357646957 created "2016-06-24" @default.
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- W2357646957 date "2012-01-01" @default.
- W2357646957 modified "2023-09-24" @default.
- W2357646957 title "Ultrastructural observations and preparation of cultured human epidermal melanocytes'samples" @default.
- W2357646957 hasPublicationYear "2012" @default.
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