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- W2357647513 abstract "Objective To observe the killing effect of peripheral blood mononuclear cells(PBMCs) irradiated by γ ray on cultured human gastric cancer cell line MKN-28.Methods The experiment were divided into MKN-28 tumor cell control group,PBMCs groups and MKN-28 cells with irradiated or non-irradiated PBMCs co-culture groups.Radidation dosage were from 0.5 to 3 Gy,acridine orange/ethidium bromide(AO/EB) staining were used to observe the kill effect of PBMCs on tumor cells in different period.Results After culture for 144h,the dead cells of several dosage irradiated PBMCs are much more than those of non-irradiated PBMCs group.At 240 hours of culture,the alive PBMCs deareses in number in both irradiated and non-irradiared groups,but decreases in radiated groups are more obvious.After culture for 72 h in the co-cultured groups,the difference is not evident among all radiation dosage groups.After 96~240 h of co-culture,the killing effect of 0.5~2Gy irradiated PBMCs on tumor cells is very strong,especially in 1Gy group,but the killing effect of PBMCs irradiated by 2.5~3Gy on tumor cells were weaker than that of 0.5~2Gy irradiated groups.At 240 hours co-cultured groups irradiated by 2.5~3Gy,tumor cells still survive and proliferate.Conclusion Gamma ray irradiation have killing effect to some PBMCs.The cytocidal effect of PBMCs irradiated by 0.5~2Gy on tumor cells were increased.Chemotaxis and cytocidal effect of tumor cells to postirradiated PBMCs were also found.The killing effect of PBMCs irradiated by 2.5 and 3Gy on tumor cells were restrained." @default.
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- W2357647513 date "2007-01-01" @default.
- W2357647513 modified "2023-09-27" @default.
- W2357647513 title "Killing Effect of Peripheral Blood Mononuclear Cells Irradiated by γ ray on Human Gastric Cancer MKN-28 cell" @default.
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