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- W2357667422 abstract "The Cry1C gene from Bacillus thuringiensis(Bt) was cloned by polymerase chain reaction(PCR).Due to a large number of E.coli low-usage codons in the gene,the first 86 bases were optimized by PCR to improve the expression in E.coli.Cry1C protein was highly expressed in E.coli BL21 as inclusion bodies,which can be dissolved in 8 mol/L urea and purified by His TrapTM FF crude column under denaturing conditions.The purified Cry1C protein was dialyzed against the refolding buffer to obtain a soluble and biologically active protein.Finally,the protein was determined to be 99.2% purity." @default.
- W2357667422 created "2016-06-24" @default.
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- W2357667422 date "2011-01-01" @default.
- W2357667422 modified "2023-09-27" @default.
- W2357667422 title "Expression in E.coli,Purification and Refolding of Recombinant Cry1C Gene from Bacillus thuringiensis" @default.
- W2357667422 hasPublicationYear "2011" @default.
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