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- W2357700737 abstract "【Objective】 To establish an indirect competitive enzyme-linked immunosorbent assay for matrine,artifical antigen was synthesized and identified.【Method】 The hapten was designed and synthesized by using sophocarpine through nucleophilic addition,diazotization and hydrogenation then characterized by MS and NMR.The Glutaraldehyde method was used to conjugate hapten to BSA and OVA,the artifical antigen was verified by SDS-PAGE,UV and IR.Polyclonal antisera were isolated from rabbits and the titer was determined by indirect non-competitive ELISA procedure.【Result】 Artificial antigen(ST) was synthesized successfully and conjugated with BSA and OVA to form immunogen(ST-BSA) and coating antigens(ST-OVA),the titer of anti-matrine serum was 128 000 after immunization of New Zealand white rabbits.【Conclusion】 Results indicated that the polyclonal antibody against matrine would be useful in the further study of IC-ELISA for matrine." @default.
- W2357700737 created "2016-06-24" @default.
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- W2357700737 date "2012-01-01" @default.
- W2357700737 modified "2023-09-25" @default.
- W2357700737 title "Synthesis and identification of artificial antigen of matrine" @default.
- W2357700737 hasPublicationYear "2012" @default.
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