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- W2357714716 abstract "Plant regeneration from protoplasts isolated from haploid cell suspensions of commer- cial supersweet maize(SS 7700)was achieved and the plants were survival after transfer into soil in pots.Protoplast plating efficiency obtained from feeder layer system was 130-fold hi- gher as compared with conventional liquid culture method,the composition of protoplast cul- ture medium,the pore size of supportive membrane filter and the relationship between proto- plasts and feeder cells were critical for callus formation.An enriched medium containing vitamins,organic acids,amino-acids and other organic substances such as coconut water could extremely improve callus formation.Filters with pore size within the range of 0.22- 8.0μm in diameter was useful.Filters with smaller pore size of 0.04μm or larger 11μm appeared to decrease the frequency of protocolony formation.The feeder cells which belong to the same species(Zea mays)as protoplasts greatly increased protoplast plating efficiencies as compared to those of feeder cells belonging to other species such as Avena nuda and Nico- tiana tabacum.Among 11 protoplast-regenerated plants examined,10plants were haploid and one plant was diploid." @default.
- W2357714716 created "2016-06-24" @default.
- W2357714716 creator A5027080891 @default.
- W2357714716 date "1989-01-01" @default.
- W2357714716 modified "2023-09-23" @default.
- W2357714716 title "PLANT REGENERATION FROM SUPERSWEET MAIZE PROTOPLASTS DERIVED FROM GAMETOPHYTIC CEL SUSPENSIONS" @default.
- W2357714716 hasPublicationYear "1989" @default.
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